2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]vinyl]-1,3,3-trimethyl-3H-indolium thiocyanate

Chemical structure

The compound has a complex structure that includes an indole ring, a vinyl group, a phenyl ring, and a thiocyanate group.

Appearance

Red-fluorescent dye.

Role

Acts as a superoxide indicator in cell biology research.

Target location

Selectively accumulates in the mitochondria of live cells.

Functionality

Upon oxidation by superoxide, exhibits red fluorescence.

Use

Valuable tool for studying oxidative stress and mitochondrial dysfunction in cellular systems.

Importance

Ability to target the mitochondria and sensitivity to superoxide helps in understanding the role of reactive oxygen species in cellular processes and diseases.

General Description

2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]vinyl]-1,3,3-trimethyl-3H-indolium thiocyanate, also known as MitoSOX Red, is a red-fluorescent dye commonly used in cell biology research as a superoxide indicator. It is structurally similar to the mitochondria-targeted probes, which enables it to selectively accumulate in the mitochondria of live cells. Upon oxidation by superoxide, MitoSOX Red exhibits red fluorescence, making it a valuable tool for studying oxidative stress and mitochondrial dysfunction in various cellular systems. The chemical's ability to target the mitochondria and its sensitivity to superoxide make it a valuable tool in understanding the role of reactive oxygen species in cellular processes and diseases.